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. 2012 Mar 26;3:52. doi: 10.3389/fpls.2012.00052

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Copyright © 2012 Gelvin.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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Interaction of GIP (GALLS Interacting Protein) with GALLS-FL and GALLS-CT. Tobacco BY-2 protoplasts were transfected with cDNAs encoding mCherry, nVenus-tagged GIP (one of the LSH protein family members), and either GALLS-FL or GALLS-CT containing a cYFP tag. The cells were imaged by epifluorescence microscopy 18 h later. mCherry marks both the nucleus and the cytoplasm. GIP-nVenus interacts with GALLS-FL-cYFP (upper row) or with GALLS-CT-cYFP (lower row) to restore fluorescence using BiFC. n Indicates the nucleus. Data courtesy of Dr. Yaling Wang.