Table A5.

List of all primers used in the present work.

Name	Direction	Sequence (5′–3′)	Tm (°C)a	Amplicon (bp)
PIN1 RT-F3	Forward	AAGCTGAAGATGGTAGGGACCTT	58	94
PIN1 RT-R3	Reverse	TGGGCGCCATAATCATGAC	59
PIN2 RT-F4	Forward	GATCAATGTTCAGGGATCAACAGA	59	81
PIN2 RT-R4	Reverse	GTTGTTGGTGGAAATGAAGTGAAA	59
PIN3 RT-F3	Forward	CTTCACGTTGCTATTGTTCAGG	54.1	238
PIN3 RT-R3	Reverse	TGACACACGACCAGCAAGTAA	56.5
PIN4 RT-F4	Forward	CGTTGGAATGAGAGGAGTGC	55	204
PIN4 RT-R4	Reverse	AATCTAAATTCCCCCTCTAATTCATGG	54.8
PIN5 RT-F2	Forward	GACTAATGCAACCAACACACCTTT	58	67
PIN5 RT-R2	Reverse	TGGATGCCGGGATATTTTACC	59
PIN6 RT-F2	Forward	CCATTCCACAAGCTGGAAATT	53.7	166
PIN6 RT-R2	Reverse	CCGGAATCTGGAGCGCCGA	62.6
PIN7 RT-F4	Forward	TCAGTGCTCGGGCATCAA	58	81
PIN7 RT-R4	Reverse	GGATCATTAGTAGATATGAAGTGGAAAGAG	58
PIN8 RT-F2	Forward	CTTCATTTGCTGTTGGACTACG	54.1	192
PIN8 RT-R2	Reverse	GTCCAAGCAAAATATAGTAAACCAGTGT	55.6
PIN9 RT-F2	Forward	GCTGCTTTTCAACCTGAATCCG	57	173
PIN9 RT-R2	Reverse	TCTGCTGCCATATCCATCTTCTTTTG	57.3
PIN10 RT-F4	Forward	GGCAGACACACCTACCCTGATC	59.4	100
PIN10 RT-R4	Reverse	CCGGAGGCATCTGTTGTTTC	56.3
PIN11 RT-F3	Forward	CAGCATTGCCACAGTCAATTACATC	56.8	196
PIN11 RT-R3	Reverse	GCCGAGCTATATTCCTCCTTCAAG	57
PIN12 RT-F6	Forward	GCTACGGCTGGTCCATTACC	58	100
PIN12 RT-R6	Reverse	ACTGCCGTCGGCCCATA	59.6
PIN13 RT-F2	Forward	GGATACATTGAGCACAGGGGTAA	56.6	199
PIN13 RT-R2	Reverse	TGGACGGGACAGACTTCTATGATTC	57.9
PIN14 RT-F3	Forward	ATAGTGATATTGTCAACAGGAGGG	54.1	175
PIN14 RT-R3	Reverse	CCAGTCTAACGGCGAAGGAAG	57.6
PIN15 RT-F2	Forward	TTTGCTGGGCTAATTTCTCAAGA	55.5	188
PIN15 RT-R1	Reverse	AGTGGGATCCCCATCACAAG	54.9
PIN16 RT-F4	Forward	GGTAACAATCTTGTCAAAGGCAGGT	57.3	199
PIN16 RT-R4	Reverse	GGATAGTTTCAACATGGTCCCTCTCA	58.2
AUX1 RT-F1	Forward	TCCCTTTATGCCAAGCTGGA	56.5	217
AUX1 RT-R1	Reverse	ATGTAGTCAGCTCACTCAGCG	56.6
AUX2 RT-F3	Forward	CGTTCGGACTCTTCGCAAAG	56.3	100
AUX2 RT-R3	Reverse	TCTTGGGACTGATTTGCTTCAG	55.1
AUX3 RT-F2	Forward	GTTCACGGCCAGGTTGATG	56.6	100
AUX3 RT-R2	Reverse	CATGCCCACCAAAAGTGTAGAG	56.1
AUX4 RT-F4	Forward	AGGGTGGGCTAGTATGTCCAA	57.7	191
AUX4 RT-R4	Reverse	AAACACAATGCAGAGGAGATGC	55.9
AUX5 RT-F1	Forward	AGCCATCAAAGTACACGGGA	56.3	174
AUX5 RT-R1	Reverse	TCTGAGGTGGGCATTGGTAA	56.1
AUX6 RT-F4	Forward	CCTGTGGTTATTCCCATTTGGTT	55.6	180
AUX6 RT-R4	Reverse	GTACTTTGGTGGTTGCTCCA	55.2
AUX7 RT-F2	Forward	CGTCAGATTGATTCATTTGGTCTATTC	54.2	213
AUX7 RT-R2	Reverse	ATCACACCTTTTCAAGAACCAACA	55.2
AUX8 RT-F1	Forward	GAGAGAATGCTGTGGAGAGAC	54.8	182
AUX8 RT-R1	Reverse	ACACTGGTAGCACTTGGTGA	56.2
ABCB1 RT-F4	Forward	GATGGTAAAGTAGCAGAGCAAGGAT	56.7	212
ABCB1 RT-R4	Reverse	ATGGGATATACTCCTCTTACTGGTGT	56.5
ABCB2 RT-F3	Forward	CAAGCATGAGACTCTGATTCATATCA	54.7	100
ABCB2 RT-R3	Reverse	AATATTGCAGGTGGTGACTCAAGA	56.4
ABCB4 RT-F2	Forward	GGGCAATCCTAAAGAATCCGAAAAT	55.7	264
ABCB4 RT-R4	Reverse	TATGAAGGGCGACCAAGGATG	56.9
ABCB5 RT-F3	Forward	TCGCAATACCTCCCGGTACA	58.1	100
ABCB5 RT-R3	Reverse	GCGTGCGGGTCGTAAAAC	57.3
ABCB7 RT-F2	Forward	GTGGTTTTGCTGTTAGATGAGGC	56.5	269
ABCB7 RT-R2	Reverse	ACTGTTTTGTGTTGTCCTCTGG	55.4
ABCB10 RT-F4	Forward	CAG AAG CAA AGG GTA GCC ATT	55.4	211
ABCB10 RT-R4	Reverse	CTCCATTTTTAACCACTGCGATTAGA	56.4
ABCB13 RT-F3	Forward	CAAGAGCAATTCTGAAAGATCCACG	56.3	206
ABCB13 RT-R3	Reverse	ACCTTTTTCCACTATCTTGCCATG	55.6
ABCB14 RT-F1	Forward	GACAGTCAAGTCAAAGAATCTCATTG	54.2	221
ABCB14 RT-R1	Reverse	TGGAACCTCTGGCTTGTTAAGA	56
ABCB13 RT-F2	Forward	CAAGAAGCACTGGACCGAATCAT	57.4	229
ABCB13 RT-R2	Reverse	TAAACACACGGAGGTGCTACAAT	56.4
ABCB18 RT-F3	Forward	AGCTCATCCATCGAATCTGAATCAA	56.3	211
ABCB18 RT-R3	Reverse	GCATCAGACGGACATACAAACCAT	57.4
ABCB19 RT-F3	Forward	TCTTAAGGACCCAGCAATCCTACT	57.3	100
ABCB19 RT-R3	Reverse	CCTCATTAGCCTCTCGAGTGCTT	58.5
ACT2 RT-F1b	Forward	GCAACTGGGATGATATGGAGA	54.3	213
ACT2 RT-R1	Reverse	TACGACCACTGGCATACAGG	56.5
UBQ RT-F1b	Forward	CAGCTTGAAGATGGGAGGAC	55.4	154
UBQ RT-R1	Reverse	CAATGGTGTCTGAGCTCTCG	55.5
TUA2 RT-F1	Forward	CCTACTGTAGTACCTGGGGGTG	58.2	230
TUA2 RT-R1	Reverse	CCAACTTCCTCGTAATCCTTCTCA	56.2
PD-E1 RT-F1	Forward	ATGAGAACTGGTGGTATTGGTGC	57.3	164
PD-E1 RT-R1	Reverse	GTCACAATCTGGGCAGGTTGAAC	58.5
CLONING AND SEQUENCING
M13F	Forward	TTGTAAAACGACGGCCAGT	54.7
M13R	Reverse	CAGGAAACAGCTATGACC	50.1
adp1-dT17c		CCGGATCCTCTAGAGCGGCCGC(T)17	64.6
adp1		CCGGATCCTCTAGAGCGGCC	61.9
PIN3 RT-F3	Forward	CTTCACGTTGCTATTGTTCAGG	54.1
PIN4 RT-F3	Forward	CTTCAGCCTCGGATAATTGTATGC	55.1
PIN11A RT-F3	Forward	GCGATGTCTTACGTGTTGCTA	55.1
PIN13 RT-F2	Forward	GGATACATTGAGCACAGGGGTAA	56.6
AUX4 RT-F3	Forward	CCGACTCCTGCAAAACATCATTA	55.4
ABCB1 RT-F3	forward	CGCATGATACAGTTACAAAGGTTCA	55.5

^aMelting temperatures were calculated with the online tool OlygoAnalyzer v.3.1 from Integrated DNA Technologies.

^bThese primer pairs have been first published in Secchi et al. (2009).

^cThis primer sequence has been first published in Kramer et al. (1998).