Table 1.

Metabolites consistently altered in all biotrophic interactions.

Metabolite	Bgh 24hpi	Bgh 96hpi	Cg 36hpi	Um 48hpi	Average
Glutamine	3.16 ± 0.52	2.19 ± 0.93	2.42 ± 0.34	2.48 ± 0.18	2.56
Glucose	1.37 ± 0.19	1.35 ± 0.16	1.43 ± 0.10	1.64 ± 0.26	1.44
Asparagine	1.81 ± 0.17	1.67 ± 0.52	2.76 ± 0.53	2.07 ± 0.10	2.07
3-PGA	−1.28 ± 0.11	−1.41 ± 0.08	−1.12 ± 0.10	−2.04 ± 0.07	−1.46
PEP	−1.25 ± 0.05	−1.37 ± 0.10	−1.25 ± 0.10	−2.08 ± 0.05	−1.48
NO. OF METABOLITE WITH F.C. > 1.5 IN INFECTED LEAVES
Metabolites increased	18 (12 – 3)	11 (3 – 0)	21 (11 – 0)	15 (12 – 11)
Metabolites decreased	3 (0 – 0)	6 (1 – 0)	2 (0 – 0)	5 (4 – 2)

Mean values of metabolite contents from four biological replicates of infected and mock control leaves harvested at the indicated time points after infection with the respective pathogens (Bgh, Blumeria graminis f.sp. hordei; Cg, Colletotrichum graminicola; Um, Ustilago maydis) were used to calculate the metabolite ratio infected/mock. Only metabolites with an average f.c. > 1.4 in both early and established biotrophic interactions are displayed (see right column). The total number of metabolites that were increased or decreased at the indicated time point of infection is indicated in the lower part of the table, with the first number in brackets giving significant changes in a t-test with p < 0.05, and the second number giving significant changes with p < 0.05 after Benjamini–Hochberg FDR correction. For individual p-values and Benjamini–Hochberg-corrected p-values, please see Table 2.