Table 3.
Percentage of up- and down-regulated genes in MapMan BINs of central primary metabolism.
| MapMan BIN | Bgh 24hpi % | Bgh 96hpi % | Um 48hpi % | Um 96hpi % | Um 108hpi % | Cg 36hpi% | Cg 96hpi % |
|---|---|---|---|---|---|---|---|
| PERCENTAGE OF UP-REGULATED | |||||||
| Starch BS | 8.1 | 0.0 | 3.3 | 23.3 | 23.3** | 0.0 | 10.0 |
| Starch Deg | 14.8 | 0.0 | 21.7** | 30.4 | 34.8 | 0.0 | 0.0 |
| Sucrose BS | 0.0 | 7.1 | 16.7 | 16.7** | 0.0 | 0.0 | 16.7 |
| Sucrose Deg | 19.2 | 11.5 | 18.5 | 18.5 | 37.0 | 0.0 | 18.5** |
| OPPP | 20.5 | 23.1 | 6.9 | 20.7 | 44.8 | 6.9 | 10.3** |
| Glycolysis | 10.1 | 7.1 | 25.8 | 29.0* | 38.7 | 9.7* | 17.7* |
| Fermentation | 27.6 | 13.8 | 26.3 | 42.1 | 52.6 | 10.5 | 21.1** |
| TCA cycle | 17.7 | 11.4 | 16.7 | 18.8 | 35.4** | 2.1 | 22.9*** |
| Calvin cycle | 8.2 | 13.7 | 10.0*** | 13.3*** | 16.7*** | 3.3** | 10.0 |
| Photorespiration | 10.0 | 16.0 | 11.9** | 9.5*** | 11.9 | 0.0 | 4.8 |
| aa Deg | 11.8 | 9.2 | 13.3** | 16.3 | 26.7* | 2.2 | 9.6 |
| Glu aa BS | 53.3*** | 46.7*** | 11.8 | 17.6 | 41.2 | 11.8 | 5.9** |
| Asp aa BS | 17.3*** | 13.5*** | 22.0 | 26.8* | 24.4 | 4.9 | 12.2 |
| bc-aa BS | 23.1* | 0.0 | 12.5 | 37.5 | 43.8 | 0.0 | 12.5 |
| Ser BS | 23.1 | 15.4** | 25.0 | 25.0** | 41.7*** | 12.5** | 25.0*** |
| aro-aa BS | 37.7*** | 43.4*** | 36.4** | 38.6 | 36.4 | 0.0 | 31.8** |
| His BS | 12.5 | 18.8*** | 12.5 | 6.3 | 6.3 | 0.0 | 6.3** |
| Nucleotides | 16.4** | 15.1*** | 13.2 | 27.2 | 41.2** | 3.5** | 14.0** |
| PERCENTAGE OF DOWN-REGULATED | |||||||
| Starch BS | 10.8** | 5.4** | 21.7* | 43.5** | 60.9** | 0.0 | 8.7 |
| Starch Deg | 3.7* | 3.7 | 16.7** | 16.7 | 30.0** | 3.3** | 6.7 |
| Sucrose BS | 0.0 | 14.3** | 33.3** | 66.7** | 66.7** | 0.0 | 16.7** |
| Sucrose Deg | 1.9 | 7.7** | 7.4 | 18.5 | 22.2 | 3.7 | 3.7** |
| OPPP | 10.3** | 2.6 | 3.4 | 20.7 | 24.1 | 0.0 | 3.4 |
| Glycolysis | 5.1*** | 4.0** | 8.1** | 11.3** | 38.7** | 0.0 | 3.2* |
| Fermentation | 3.4 | 3.4 | 15.8* | 31.6** | 26.3** | 0.0 | 5.3 |
| TCA cycle | 1.3** | 1.3*** | 2.1** | 2.1** | 12.5** | 0.0 | 2.1** |
| Calvin cycle | 11.0*** | 6.8** | 16.7* | 56.7** | 66.7*** | 3.3** | 6.7** |
| photorespiration | 6.0*** | 0.0 | 14.3* | 31.0** | 45.2** | 2.4** | 31.0 |
| aa Deg | 6.5*** | 2.6** | 10.4*** | 21.5** | 22.2** | 0.7 | 6.7 |
| Glu aa BS | 0.0 | 0.0 | 17.6 | 17.6 | 11.8 | 0.0 | 5.9* |
| Asp aa BS | 0.0 | 1.9 | 9.8 | 12.2 | 14.6 | 0.0 | 4.9 |
| bc-aa BS | 7.7 | 7.7 | 12.5 | 18.8 | 56.3 | 0.0 | 12.5 |
| Ser BS | 0.0 | 0.0 | 12.5** | 16.7* | 33.3** | 0.0 | 0.0 |
| aro-aa BS | 3.8 | 1.9 | 2.3 | 13.6 | 15.9 | 6.8 | 11.4** |
| His BS | 0.0 | 0.0 | 6.3 | 6.3 | 12.5 | 0.0 | 6.3 |
| Nucleotides | 4.8** | 2.7** | 8.8** | 14.0 | 15.8** | 0.0 | 2.6* |
The percentage of up- (upper half) and down-regulated genes (lower half) in the indicated MapMan BINs was calculated based on the mean fold changes from all three replicate experiments. Strong differences between the two time points of established biotrophy, Bgh 96hpi and Um 48hpi, are indicated in bold. Significant enrichment of MapMan BINs was calculated with a Wilcoxon rank sum test: *p < 0.1 and **p < 0.05. ***indicates p < 0.05 in a Wilcoxon rank sum test after Benjamini–Hochberg FDR correction. BS, biosynthesis; Deg, degradation; aro-aa, aromatic amino acids; bc-aa, branched-chain amino acids; OPPP, oxidative pentose phosphate pathway.