Table 4.
Consistent transcriptional changes among the pathosystems.
| Gene | Probe set Barley1 | Bgh 24hpi | Bgh 96hpi | Probe set Maize Affy | Cg 96hpi | Um 48hpi | Classification |
|---|---|---|---|---|---|---|---|
| TCA CYCLE/CARBOXYLATE METABOLISM | |||||||
| Citrate lyase | Contig3815_at | 2.1 | Zm.1942.1.A1_at | 4.4 | Early | ||
| Aconitase | Contig3351_s_at | 1.5 | Zm.12697.1.S1_at | 2.1 | 2.6 | Global | |
| Oxoglutarate dehydrogenase | Contig4963_at | 1.6 | Zm.6807.1.A1_at | 5.0 | 2.0 | Global | |
| Cytosolic malate dehydrogenase | Contig3610_s_at | 1.9 | 3.5 | Zm.2061.1.A1_at | 3.0 | 4.0 | Global |
| NAD malic enzyme | HV_CEb0015P21f_S_at | 1.8 | 1.5 | Zm.3666.1.A1_at | 1.9 | Global | |
| Pyruvate decarboxylase | Contig5532_s_at | 1.7 | Zm.3994.1.S1_at | 10.2 | Late | ||
| NUCLEOTIDE METABOLISM | |||||||
| Adenosine/uridine kinase | Contig2829_at | 1.7 | 1.9 | Zm.247.2.A1_at | 5.2 | 32 | Global |
| UMP synthase | Contig16393_at | 1.9 | 2.3 | Zm.908.1.A1_at | 3.0 | Global | |
| Nucleoside diphosphosphate | Contig2124_at | 2.3 | Zm.19303.1. | 27 | 4.1 | Global | |
| kinases* | S1_at (Zm.17247.1. A1_at) | ||||||
| AMINO ACID METABOLISM | |||||||
| Serine | Contig2168_s_at | 1.8 | 2.6 | Zm.3136.1.A1_at | 2.4 | Globalhydroxymethyltransferase | |
| Methylene etrahydrofolate reductase | Contig3235_s_at | 1.8 | Zm.475.1.S1_at | 1.9 | Earlyt | ||
| Amino acid transporters | Contig26356_at | 1.5 | 1.5 | Zm.1788.1.A1_at | – | 2.6 | Global |
| Aminotransferases** | Contig1672_s_at | −2.5 | −1.8 | Zm.13511.1.A1_at | 1.5 | −4.2 | Global biotr |
| Zm.2321.1.A1_at | |||||||
| Glutamine synthetase | Contig1646_at | −1.7 | Zm.3455.3.A1_at | −1.6 | Early | ||
| PRPP synthetase | Contig8025_at | −5.3 | 2.3 | Zm.1727.1.A1_at | −2.0 | 1.6 | Early < to > late |
| SUCROSE METABOLISM | |||||||
| PFK2 | 1.5 | Zm.711.1.S1_at | 3.0 | Late | |||
| H+/PPase | Contig385_s_at | −2.0 | Zm.6095.1.A1_at | 2.1 | 4.3 | ||
| STARCH METABOLISM/CALVIN CYCLE | |||||||
| AGPase | Contig5267_at | 1.6 | Zm.312.1.A1_at | 1.9 | Early | ||
| Phosphoribulokinase | rbaal2124_s_at | −1.8 | Zm.2248.1.A1_at | −2.5 | |||
| Aldolase | Contig4817_at | 1.8 | Zm.4778.1.A1_at | 2.5 | Late | ||
Transcriptional changes during biotrophic Bgh colonization (early stage – Bgh 24hpi; late stage Bgh 96hpi), necrotrophic Cg colonization (Cg 96hpi), and biotrophic Um colonization (Um 48hpi) is compiled. Fold changes were calculated based on mean values from all three replicate experiments, respectively. Only genes with f.c. > 1.5 are displayed that are regulated in more than one pathosystem.
*For Cg 96hpi, data for adenylate kinase (Zm.17247.1.A1_at) are given.
**For Bgh, and Cg (Zm.13511.1.A1_at), the induced aminotransferases are annotated as aspartate glutamate aminotransferases, while the Um induced aminotransferase (Zm.2321.1.A1_at) is supposed to be an alanine oxoglutarate aminotransferase. Both of these aminotransferase activities are associated with central nitrogen metabolism.