Figure 6.

The N-terminal 43 amino acids of SFD1 are required for targeting SFD1 to the chloroplast. (A) SAR conferred resistance against P. syringae pv. maculicola (Pma) in the wild type (WT) Arabidopsis accession Nössen plant, and in transgenic sfd1-1 mutant plants that express GFP or the SFD1–GFP and SFD1Δ43–GFP constructs in which the GFP coding sequence is fused in frame to the C-terminus of the SFD1 protein. Refer to legend to Figure 4B on details for the induction of SAR. An *indicates significant (P < 0.05) differences in Pma numbers in plants that were previously treated with an avirulent pathogen (black bars) relative to plants that received a prior mock treatment (white bars). (B) The green fluorescence of GFP and red autofluorescence of chloroplasts were observed in protoplasts isolated from leaves of 4-week-old sfd1-1 mutant, and transgenic sfd1-1 mutant plants expressing GFP, SFD1–GFP, and SFD1Δ43–GFP. Merged images of green fluorescence of GFP and red autofluorescence of chloroplasts are also depicted.