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DHAP reductase activity of WT and mutant SFD1 proteins.
| SFD1 construct | Km (μM) | Vmax (μmol mg−1 min−1) |
|---|---|---|
| SFD1-WT | 0.33 ± 0.16 | 49.24 ± 4.58 |
| SFD1Δ43 | 0.20 ± 0.05 | 41.11 ± 1.93 |
| SFD1–Ala194 | nd | nd |
| SFD1–Ala279 | nd | nd |
| SFD1–Ala332 | nd | nd |
nd, No activity detected.
