Table 2.
Primer name | Sequence 5′ → 3′ |
---|---|
SFD1-a1 | CCTTTTATATCTCTTAGCGCGGGTCTGGAGCTTAATACTC |
SFD1-a2 | GAGTATTAAGCTCCAGACCCGCGCTAAGAGATATAAAAGG |
SFD1-b1 | GAAATCGCCGGTGCCCTGGCGAATGTTCTAGCAATAG |
SFD1-b2 | CTATTGCTAGAACATTCGCCAGGGCACCGGCGATTTC |
SFD1-c1 | GTTTATCAGGAACTGGGGCGATAATGCTTACGTGTTTTG |
SFD1-c2 | CAAAACACGTAAGCATTATCGCCCCAGTTCCTGATAAAC |
SDF1ΔNt43-F1 | GGATCCGAATTCATCTCTGGTCCGCCTG |
SDF1ΔNt43-R1 | AAGCTTGTCGACTCATACTTCTTCAATCTGAGG |
SDF1ΔNt43-F2start | ATCTCTGGTCCGCCTGATATC |
SDF1ΔNt43-R2stop | TCATACTTCTTCAATCTGAGGA |
SDF1ΔNt43-R2Ct | TACTTCTTCAATCTGAGGAAG |
SFD1-Fstart | ATGGCGGCTTCGGTGCAACCTGC |
SFD1-Rstop | TCATACTTCTTCAATCTGAGGA |
SFD1-RCt | TACTTCTTCAATCTGAGGAAG |
T7-F | TAATACGACTCACTATAGGG |
T7-R | TAGTTATTGCTCAGCGGTGG |
Hyg-F | GATGTTGGCGACCTCGTATT |
Hyg-R | GATGTAGGAGGGCGTGGATA |
attR1-F | TTGGAGAGGACCTCGACTCT |