Table 1.
Phosphorylated MP17 peptides.
Position [aa] | Score | Sequence | Digestion |
---|---|---|---|
69–76 | 42 | R.TTSWATPK.E | Trypsin |
77–89 | 33 | K.EVSPSGRVYQTVR.H | Trypsin |
133–144 | 46 | K.YHPSSPTSTSSK.L | Trypsin |
69–76 | 32 | R.TTSWATPK.E | Trypsin/chymotrypsin |
77–85 | 27 | K.EVSPSGRVY.Q | Trypsin/chymotrypsin |
133–144 | 24 | K.YHPSSPTSTSSK.L | Trypsin/chymotrypsin |
133–144 | 21 | K.YHPSSPTSTSSK.L | Trypsin/chymotrypsin |
Sequence of identified MP17-phosphopeptides (after digestion with trypsin alone or trypsin followed by incubation with chymotrypsin) is separated from flanking amino acids by dots and phosphorylated serines are given in bold; score-values calculated by MASCOT [Score = -10 × Log(p)] are a means of expressing probability p that a match of theoretical and observed mass is by chance, where a score of 20 accounts for p ≤ 0.01.