Table 2.
Primer used in this study.
| Construct | Primer | Sequence 5'–3' |
|---|---|---|
| MP17-PMH | FV17 | GGTACCATGTCAATGGTGGTGTACAACAACC |
| FV20 | TATAGGATCCTCAGTGATGGTGATGGTGATGTCCGCGCTTGATAAGTTTTGGC | |
| DELETIONS | ||
| MP17fl | KL1 | CACCAACAATGTCAATGGTGGTGTACAACAACCAAGG |
| KL2 | TCCGCGCTTGATAAGTTTTGGC | |
| MP17Δ 1-18 | KL3 | CACCAACAATGGGCGCGCTAACAGAGTTCAGCC |
| KL2 | TCCGCGCTTGATAAGTTTTGGC | |
| MP17Δ 110-156 | KL1 | CACCAACAATGTCAATGGTGGTGTACAACAACCAAGG |
| KL4 | GAAGTAAGATGCTTGTGATCTTATACTCATGG | |
| MP17Δ 1-109 | KL7 | CACCAACAATGAGTTCGTCAGCGAGG |
| KL2 | TCCGCGCTTGATAAGTTTTGGC | |
| MP17Δ 1-99 | KL20 | CACCAACAATGATGAGTATAAGATCACAAGCATCTTACTTC |
| KL2 | TCCGCGCTTGATAAGTTTTGGC | |
| MP17Δ 1-89 | KL21 | CACCAACAATGCATTCAAGGATGGAATACTCAAGGC |
| KL2 | TCCGCGCTTGATAAGTTTTGGC | |
| MP17Δ 1-79 | KL22 | CACCAACAATGCCTTCGGGCCGAGTCTATCAG |
| KL2 | TCCGCGCTTGATAAGTTTTGGC | |
| MP17Δ 1-66 | KL23 | CACCAACAATGCAAAGGACAACCTCATGGGCAAC |
| KL2 | TCCGCGCTTGATAAGTTTTGGC | |
| MP17Δ 1-53 | KL24 | CACCAACAATGTTCCCCGAGGACGAGGC |
| KL2 | TCCGCGCTTGATAAGTTTTGGC | |
| MP17Δ 1-40 | KL25 | CACCAACAATGGTAGAAGAGGAGGCAATCGCCG |
| KL2 | TCCGCGCTTGATAAGTTTTGGC | |
| MP17Δ 1-27 | KL26 | CACCAACAATGTTATGGTCACGGCCTCTGGG |
| KL2 | TCCGCGCTTGATAAGTTTTGGC | |
| SUBSTITUTIONS | ||
| MP17A69-81 A | KL1 | CACCAACAATGTCAATGGTGGTGTACAACAACCAAGG |
| KL28 | AGTCTGATAGACTCGGCCCGCAGGTGCAACTTCCTTGGGAGCTGCCCATGCGGCTGCCCTTTGTAAACACGAATGTC | |
| MP17A69-81 B | KL27 | GACATTCGTGTTTACAAAGGGCAGCCGCATGGGCAGCTCCCAAGGAAGTTGCACCTGCGGGCCGAGTCTATCAGACT |
| KL2 | TCCGCGCTTGATAAGTTTTGGC | |
| MP17A136-143 A | KL1 | CACCAACAATGTCAATGGTGGTGTACAACAACCAAGG |
| KL13 | CGCCGCCCTTCGTAATTTGGCAGCCGCGGCCGCAGGAGCGGCGGGATGATACTTTGCAATGGG | |
| MP17A136-143 B | KL12 | CCCATTGCAAAGTATCATCCCGCCGCTCCTGCGGCCGCGGCTGCCAAATTACGAAGGGCGGCG |
| KL2 | TCCGCGCTTGATAAGTTTTGGC | |
| MP17Aall | Template MP17A136-143, primers as MP17A69-81 | |
| MP17D71 A | KL1 | CACCAACAATGTCAATGGTGGTGTACAACAACCAAGG |
| KL30 | CTTGGGAGCTGCCCAGTCGGCTGCCCTTTGTAA | |
| MP17D71 B | KL29 | TTACAAAGGGCAGCCGACTGGGCAGCTCCCAAG |
| KL2 | TCCGCGCTTGATAAGTTTTGGC | |
| MP17D79 A | KL1 | CACCAACAATGTCAATGGTGGTGTACAACAACCAAGG |
| KL32 | GACTCGGCCCGCAGGGTCAACTTCCTTGGGAGC | |
| MP17D79 B | KL31 | GCTCCCAAGGAAGTTGACCCTGCGGGCCGAGTC |
| KL2 | TCCGCGCTTGATAAGTTTTGGC | |
| MP17D7179 | template MP17D71, primers as MP17D79 | |
| Primer to merge A and B | KL1 | CACCAACAATGTCAATGGTGGTGTACAACAACCAAGG |
| KL2 | TCCGCGCTTGATAAGTTTTGGC | |
| MP17Δ110-156D71 | template MP17D71, primers as MP17Δ 110-156 | |
Topo directional site and enhancer sequence are underlined, triplets encoding for six histdines are written in italic