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. 2012 Apr 11;32(15):5074–5084. doi: 10.1523/JNEUROSCI.5808-11.2012

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Copyright © 2012 the authors 0270-6474/12/325074-11$15.00/0

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Figure 2. — Inducing SMN rescues Pls3 expression in smn mutants. A, Schematic diagram of the 1.5 kb zebrafish heat shock 70 promoter (hsp70) controlling expression of RFP tagged human SMN (RFP-SMN) transgene and a 2 dpf Tg(hsp70:RFP-SMN) embryo 1 d after heat shock (hs). B, RFP-SMN protein levels after heat shock induction. Lane 1, 1 dpf embryo preinduction; lanes 2–6, 2–6 dpf larvae after induction (hs) at 1 dpf; lanes 7 and 8, 7–8 dpf larvae after induction (hs) again at 6 dpf; lane 9, 9 dpf larvae after induction (hs) again at 8 dpf. C, Western blot for endogenous Smn and Pls3 in wild-type (WT), smn heterozygous (smn^+/−), and smn homozygous (smn^−/−) mutants at 8 dpf. D, Western blot of protein from 8 dpf Tg(hsp70:RFP-SMN) larvae after heat shocking at 5 and 7 dpf. Pls3, RFP-SMN, Smn, and β-Actin were analyzed in WT, Tg(hsp70:RFP-hSMN), Tg(hsp70:RFP-hSMN);smn^+/−, and Tg(hsp70:RFP-hSMN);smn^−/−.