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. 2012 Apr 11;32(15):5074–5084. doi: 10.1523/JNEUROSCI.5808-11.2012

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Copyright © 2012 the authors 0270-6474/12/325074-11$15.00/0

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Figure 8. — Adding PLS3 back to motoneurons rescues NMJ defects in smn^−/− larvae. A, Lateral view of 11 dpf. Left panel, Tg(mnx1:PLS3); middle panel, Tg(mnx1:GFP);smn^−/−; and right panel, Tg(mnx1:PLS3);smn^−/− (n = 8 fish for each group) immunolabeled with anti-SV2. B, Quantification of the number of pixels per area (A, white box) using confocal microscopy and MetaMorph software. Six myotomes were measured per fish. ***p < 0.0001 for smn^−/− versus Tg(mnx1:PLS3);smn^−/− (Student's t test). Scale bar, 80 μm.