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. 2012 Apr 5;3:66. doi: 10.3389/fpls.2012.00066

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Copyright © 2012 Juntawong and Bailey-Serres.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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Quantitative analysis of the molecular response to the shift to early darkness and re-illumination in Arabidopsis (Col-0) seedlings. (A) Experimental scheme of the light cycle used for seedling growth for 14 days on MS medium supplemented with 1% (w/v) sucrose. Arrows indicate time of day relative to the beginning of the light period (ZT0). Light (control) seedlings were harvested in the light at ZT9, Early Dark seedlings were transferred to darkness at ZT8 for 1 h and harvested, and Re-illumination seedlings were transferred to darkness at ZT8 for 1 h and then re-illuminated for 10 min before harvest. (B) Flow diagram of experimental strategy for quantitative assessment of transcript abundance in the total mRNA and immunopurified polysomal mRNA populations.