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. 2012 Feb 2;3:14. doi: 10.3389/fpls.2012.00014

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Copyright © 2012 Anderson, Wang, Bandyopadhyay and Goodin.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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Single-plane confocal micrographs of GFP: protein fusions expressed transiently in leaf epidermal cells of N. benthamiana plants transgenic for the nuclear marker, RFP: histone 2B. Shown in panels 1, 2, and 3 are micrographs of GFP (fusions), RFP, and the resultant overlay, respectively. (A1–A3) GFP only. (B1–B3) PYDV-N. (C–L) PYDV-N site-directed double mutants. Numbers indicate the amino acid residue in the full-length N protein where a two-residue change to alanine was introduced. Changes to glycine were made in cases where the residue was alanine in the native protein. Scale bar = 10 μm.