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. 2012 Feb 2;3:14. doi: 10.3389/fpls.2012.00014

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Copyright © 2012 Anderson, Wang, Bandyopadhyay and Goodin.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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Yeast-based assay for identification of proteins containing a functional NLS. (A) Positive [histone 2B (H2B)] and negative (GFP and GST) control proteins along with PYDV proteins (N, and site-directed N mutants) were expressed from pNIA–DEST in yeast strain L40. Only proteins containing a functional NLS are able to facilitate yeast growth on media lacking histidine. (B) Localization of the karyophilic domain of PYDV-N (aa 419–434; NLS) fused to NIA transcriptional activator. Introducing the QK431AA mutation into this domain abolished nuclear import activity.