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. 2012 Feb 2;3:14. doi: 10.3389/fpls.2012.00014

Figure 6.

Figure 6

Confocal micrographs of BiFC assays testing the ability of PYDV-N mutant proteins to associate with the wild-type N protein. Interaction assays were conducted in leaf epidermal cells of transgenic N. benthamiana expressing a fluorescent nuclear marker (Nucleus). Panels 1, 2, and 3 show micrographs of YFP (BiFC), nuclear marker, and the resultant overlay, respectively. Shown here are BiFC assays conducted with wild-type PYDV-N expressed as a fusion to the carboxy-terminal portion of YFP and N mutants expressed as fusions to the amino-terminal fragment of YFP. (A) GST, used as a non-binding control. (B) Wild-type PYDV-N. (C–L) BiFC between PYDV-N and site-directed double mutants of PYDV-N shown in Figure 1. Scale bar = 10 μm.