Figure 7.

Confocal micrographs showing results of BiFC assays to determine the ability to self-associate. Interaction assays were conducted in leaf epidermal cells of transgenic N. benthamiana expressing a fluorescent nuclear marker (Nucleus). (A–C) GST, as a non-binding control. (D–F) Self-interaction of wild-type PYDV-N. (G–I) BiFC between PYDV-N and an N mutant that is incapable of nuclear import. (J–L) Self-interaction of QK419AA mutant of PYDV-N. All N-NLS mutants were found to retain the ability to self-interact (data not shown). Scale bar = 10 μm.