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. 2012 Feb 2;3:14. doi: 10.3389/fpls.2012.00014

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Copyright © 2012 Anderson, Wang, Bandyopadhyay and Goodin.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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Confocal micrographs showing PYDV-N fragment interactions with PYDV-N determined by BiFC. Interaction assays were conducted in leaf epidermal cells of transgenic N. benthamiana expressing a fluorescent nuclear marker (Nucleus). Shown is the ability of fragments of PYDV-N to interact with wild-type N protein in BiFC assays. (A1–A3) GST. (B1–B3) Wild-type PYDV-N. (C–H) BiFC between PYDV-N and PYDV-N fragments F1–F6. The portions of PYDV-N used in the BiFC assays are indicated by the amino acid (aa) designation for each panel. Scale bar = 10 μm.