Figure 9.

Confocal micrographs showing PYDV-N mutant protein interactions with PYDV-P determined by BiFC. Interaction assays were conducted in leaf epidermal cells of transgenic N. benthamiana expressing a fluorescent nuclear marker protein fusion. Panels 1, 2, and 3 show micrographs of YFP (BiFC), nuclear marker (Nucleus), and the resultant overlay, respectively. All interactions were tested against PYDV-P expressed as a fusion to the carboxy-terminal of YFP. (A1–A3) Non-binding control GST. (B1–B3) BiFC between PYDV-P and PYDV-N. (C–L) BiFC between PYDV-P and double mutants of PYDV-N. Scale bar = 10 μm.