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Pregnenolone formation from cholesterol in the quail brain. Biochemical analysis of the formation of pregnenolone from cholesterol using intact mitochondria derived from the quail brain. Incubations were performed with 200 μM cholesterol, 0.25 mg of mitochondrial protein, 10 mM isocitrate and 10 mM succinate at 41°C for different times. See Tsutsui and Yamazaki (1995) for details.
