Figure 6.

Androgen formation from androstenedione in the quail brain. HPLC analysis of neurosteroids extracted from quail diencephalic slices after different incubation times with ³H-androstenedione using a reversed-phase column. The column was eluted with a 30-min linear gradient of 40–70% acetonitrile, followed by an isocratic elution of 70% acetonitrile. The ordinate indicates the radioactivity measured in each HPLC fraction. The arrows indicate the elution positions of androstenedione and testosterone. See Matsunaga et al. (2002) for details.