Figure 7.

Estrogen formation from androgen in the quail brain. HPLC analysis of neurosteroids extracted from quail diencephalic slices after 60 min incubation with ³H-progesterone using a reversed-phase column. The column was eluted with a 30-min linear gradient of 40–70% acetonitrile, followed by an isocratic elution of 70% acetonitrile. The ordinate indicates the radioactivity measured in each HPLC fraction. The arrows indicate the elution positions of progesterone, androstenedione, testosterone, and estradiol-17β. See Matsunaga et al. (2002) for details.