Figure 2.

Opposite effect of MEHP on FSH and hCG-stimulated steroidogenesis in immature granulosa cells (A) and Leydig cell progenitors (B). The steroidogenic cells were cultured without or with MEHP at different concentrations for 24 h and then incubated with hCG (10 ng/ml) and FSH (50 mU/ml) in the absence or presence of the same concentrations of MEHP for additional 24 h. Testosterone (T) and 3α-Adiol and progesterone in the culture medium were then measured by RIA. The mean values ± SEM for four independent steroidogenic cell preparations are presented. *p < 0.05 compared to treatment with FSH and hCG.