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The A(133)S SNP induces enhanced adhesion. (A) H1299 cells stably transfected with HA tagged RASSF1A expression vector were assayed for the strength of their adhesion to culture dishes. The difference between the wild type and the A(133)S variant transfected cells was statistically significant (P = 0.03). (B) Levels of RASSF1A expression in the stable cell lines was confirmed by western analysis using an HA antibody.
