Table 3.
Summary of proteins identified as demonstrating differential interactions with the wild type and A(133)S variant of RASSF1A.
| Protein target | RASSF1AWT | RASSF1AA133S |
|---|---|---|
| Tau | 0.26 | 0 |
| AGR3/BCMP11 | 0 | 0.5 |
| ANAPC7 | 0 | 0.15 |
| Claudin 10 | 4 | 0 |
| Vimentin | 0.55 | 0 |
| RASSF3 | 0.36 | 0 |
| Map1a | 0.68 | 0 |
| Ksr | 0.07 | 0.0 |
| MST1 | 0.1 | 0.1 |
| C190RF5 | 0.7 | 0.7 |
| RASSF1A | 4.3 | 3.8 |
Quantification of interactions is shown as protein abundance factor (PAF). Essentially this value is calculated from the total of unique MS/MS spectra that identified each individual protein divided by the molecular weight (Dalton) × 104. Proteins denoted in blue are proteins that are already known to bind directly to RASSF1A. Therefore, their presence in the screen at equivalent levels serves as an internal control. The levels of RASSF1A expressed during the transfection can also be seen to be similar (denoted in red).