Figure A3.

Lack of SUMOylation at K249 does not alter Aurora-A dynamics at centrosomes during interphase. HeLa cells stably expressing EGP–AurkA variants were used in a series of fluorescence recovery after photobleaching (FRAP) experiments. The upper panels show representative images of Aurora-A^WT and Aurora-A^K249R recovery at centrosomes during interphase. A graph shows the half-lives obtained using regression analysis to integrate data from 10 cells each. The half-lives of EGFP–Aurora-A variants at interphase centrosomes were not different when compared using a unpaired t-test.