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. 2011 Sep 20;2:30. doi: 10.3389/fendo.2011.00030

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Copyright © 2011 Kittilson, Jones and Sheridan.

This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.

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Growth hormone (GH) activates multiple signal pathways via rainbow trout GH receptors (GHR) transfected in Chinese hamster ovary (CHO)-K1 cells. CHO-K1 cells were stably transfected with cDNA encoding rainbow trout GHR1 and GHR2. Cells were incubated with 100 ng/ml salmonid GH for various times; after which, the cells were collected and lysed. Lysates were separated by SDS-PAGE followed by Western immunoblotting using phospho-specific (pERK 1/2, pAkt, pJAK2, pSTAT5) and total (tERK 1/2; tAkt, tJAK2, tSTAT5) antibodies.