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. 2012 Jan 9;2:114. doi: 10.3389/fendo.2011.00114

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Copyright © 2012 Huang, Bellés and Lee.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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(A) Alignment of the deduced amino acid sequences of the Blattella germanica HTH receptor (Blage-HTHR, GenBank GU591493) with the Periplaneta americana AKH receptor (Peram-AKHR, GenBank ABB20590). Identical amino acid residues are highlighted in gray. The seven transmembrane α-helices are indicated by TM1-7. The dotted-line rectangle indicates an insertion present in both cockroach species. (B) Neighbor joining tree of AKH receptor insect sequences. The tree was generated using MEGA 5 with 1000 bootstrap replicates. The evolutionary distance is given in units of the number of amino acid substitutions per site. The abbreviated names of insect AKHR are: Acypi-AKHR (Acyrthosiphon pisum), Aedae-AKHR-1 and Aedae-AKHR2 (Aedes aegypti), Anoga-AKHR (Anopheles gambiae), Apime-AKHR (Apis mellifera), Blage-HTHR (Blattella germanica), Bommo-AKHR (Bombyx mori), Drome-AKHR (Drosophila melanogaster), Glomo-AKHR (Glossina morsitans), Manse-AKHR (Manduca sexta), Nasvi-AKHR (Nasonia vitripennis), Pedhu-AKHR (Pediculus humanus), Peram-AKHR (Periplaneta americana), and Trica-AKHR (Tribolium castaneum). Gonadotropin-releasing hormone receptor of Caenorhabditis elegans (Caeel-GnRHR) was used as outgroup.