Figure 2.

(A) Semiquantitative RT-PCR analysis (Blage-HTHR, 790 bp) in various tissues: brain–CC–CA complex (B–CC), ventral nerve cord (VNC), midgut (Mgt), fat body (FB), ovary (O), oviduct (Ovd), and accessory gland (Asg) of 7-day-old adult females. BgActin expression (365 bp) was used as a reference. (B) Quantitative real-time RT-PCR analysis of Blage-HTHR expression in the whole body of cockroaches at various developmental stages. N1 and N6 represent first and last (sixth) nymphal instars, respectively. Male and female are 7-day-old adults. The Blage-HTHR mRNA was normalized against BgActin expression. Data represent the mean ± SEM (n = 3) of relative transcription levels normalized in comparison with embryo.