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. 2012 Jan 9;2:114. doi: 10.3389/fendo.2011.00114

Figure 3.

Figure 3

Effects of silencing Blage-HTHR on hemolymph trehalose levels in Blattella germanica. dsHTHR or dsEGFP were injected in newly emerged adults. (A) Semiquantitative RT-PCR analysis showing the down-regulation of Blage-HTHR expression in the fat body 1 day after injection of the corresponding dsRNA. (B) Silencing effect of Blage-HTHR on hemolymph trehalose levels after treatment with HTH (10 pmol; n = 16) 0 and 2 h after HTH treatment. *Indicates significant differences between 0 and 2 h after HTH treatment (P < 0.05, two-tailed Student’s t-test). (C) Pattern of hemolymph trehalose in dsHTHR-treated adult females during the first reproductive cycle (n = 8, except at the age of 4 days, where n = 4). (D) Pattern of hemolymph trehalose in dsHTHR-treated adult males (n = 6). *P < 0.05 is using two-tailed Student’s t-test.