Figure 5.

Effects of double RNAi of Blage-HTH and Blage-HTHR against oxidative stress in male adults of Blattella germanica. (A) The effect of double injection of dsHTH and dsHTHR on the gene expression 10 days after the treatment. (B) Effect of Blage-HTH and Blage-HTHR RNAi on survival rate under PQ treatment. E, injection of dsEGFP; H, double injection of dsHTH and dsHTHR on newly emerged adults. SA, PQ, and 40H as described in Figure 3. The median survival time (days) is indicated in the parenthesis of each treatment group. (C) Lipid peroxidation 4 h after PQ treatment. MDA, malondialdehyde as an indicator of lipid peroxidation. Results are presented as the mean ± SEM (n = 4 per treatment group). Different letters on the histogram indicate significant differences within the same dsRNA treatment. Two-tailed t-test was performed between different dsRNA injections.