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. 2011 Jan 14;1:12. doi: 10.3389/fendo.2010.00012

Figure 4.

Figure 4

Comparison of luminescence intensities of different luciferase-tagged OxR2 fusion proteins over time oxidizing different luciferase substrates: coelenterazine h and DeepBlueC. HEK293FT cells were transiently co-transfected with OxR2 tagged with various luciferase constructs and β-arrestin 2/Venus (A) or β-arrestin 2/GFP10 (B). Luciferase substrates were added immediately prior to real-time measurement at 37°C. Cells were assayed before and after treatment with vehicle (data for which are shown in this figure) or OxA (data not shown). These data were collected as part of the BRET assay and were also used for calculation of the corresponding BRET ratio presented in Figure 5. Results shown are representative of three independent experiments.