Table 1.

Relative luminescence intensities of the three V2R mutants investigated compared with the luminescence intensity of the corresponding V2R-wild type construct (V2R-wild type/Rluc8 or V2R-wild type/Rluc).

Transfected luminophore construct	Luminescence relative to corresponding V2R-wild type/luminophore (%)
V2R-R137C/Rluc8	84.9 ± 3.1
V2R-R137L/Rluc8	101.0 ± 4.1
V2R-R137H/Rluc8	77.7 ± 3.4
V2R-R137C/Rluc	129.2 ± 8.8
V2R-R137L/Rluc	127.2 ± 11.1
V2R-R137H/Rluc	100.3 ± 8.7

Luminescence intensities were detected as part of the BRET kinetic assay, with the cells being co-transfected with β-arrestin 2/Venus.

Data shown are mean ± SEM of four independent experiments.

Data for Rluc8-tagged constructs have been published previously (Kocan et al., 2009) and are included for comparison.