Table 2.
Primer sequences.
| MGMT | Forward seq | Tm/position | Ref NCBI | Chromosome/position | Reverse seq | Tm/position | Ref NCBI | Chromosome/position | ||
| 1PCR | GTTTYGGATATGTTGGGATAGT | 52.6 | NT 008818.16 | 10 | 2499402–2499423 | AATAAAAACRCCTACAAAACCACTC | 53.6 | NT 008818.16 | 10 | 2499558–2499581 |
| 2PCR | GTTTYGGATATGTTGGGATAGT | 52.6 | NT 008818.16 | 10 | 2499402–2499423 | Biot-AAAACCACTCRAAACTACCACC | 54.6 | NT 008818.16 | 10 | 2499546–2499567 |
| Seq primer | GGATATGTTGGGATAGTT | 44.1 | NT 008818.16 | 10 | 2499407–2499424 | |||||
| CRABP1 A | Forward seq | Tm | Reverse seq | Tm | ||||||
| 1PCR | AGTTGTTTAAGGTATTGGGTAAG | 58.2 | NT 010194.17 | 15 | 49423380–49423401 | AAACTCCCCAACTTCCACC | 61.4 | NT 010194.17 | 15 | 49423483–49423500 |
| 2PCR | AGGTATTGGGTAAGTTGGTGTAG | 61.5 | NT 010194.17 | 15 | 49423389–49423411 | Biot-CAACTTCCACCCCCTCCA | 62.1 | NT 010194.17 | 15 | 49423475–49423492 |
| Seq primer | GGGTAAGTTGGTGTAGA | 46.7 | NT 010194.17 | 15 | 49423396–49423412 | |||||
| MLH1 (lower strand) | Forward seq | Tm | Reverse seq | Tm | ||||||
| 1PCR | TGGGGTTTTGTGTTTGGTT | 58.2 | NT_022517.18 | 3 | 36974265–36974289 | AACCTACACRAACAACTCTCTCTTC | 57.9 | NT_022517.18 | 3 | 36974446–36974464 |
| 2PCR | TGGGGTTTTGTGTTTGGTT | 58.2 | NT_022517.18 | 3 | 36974313–36974335 | Biot-AATCRCCCTAACRCAAACRCTCCACCAAAAC | 56.4 | NT_022517.18 | 3 | 36974446–36974464 |
| Seq primer | GTTATTATGAGGTTGAGT | NT_022517.18 | 3 | 36974385–36974402 | ||||||
| CACNA1G | Forward seq | Tm | Reverse seq | Tm | ||||||
| 1PCR | GGAAGAGGGGGYGTTTTTTTT | 55.9 | NT_010783.15 | 17 | 13912553–13912573 | TCCCCTACRCCCCTAAAACTT | 55.9 | NT_010783.15 | 17 | 13912723–13912743 |
| 2PCR | Biot-GGAAGAGGGGGYGTTTTTTTT | 55.9 | NT_010783.15 | 17 | 13912553–13912573 | CTCCCCCTCACTTTATTC | 54.5 | NT_010783.15 | 17 | 13912687–13912704 |
| Seq primer | CCCCTCACTTTATTCC | 36.7 | NT_010783.15 | 17 | 13912686–13912701 | |||||
| NEUROG1 | Forward seq | Tm | Reverse seq | Tm | ||||||
| 1PCR | GTATATTTYGGGTTGGGGGAGG | 55.8 | NT_34772.6 | 5 | 43185718–43185697 | CCCTCCCTTACCACCTC | 59.3 | NT_34772.6 | 5 | 43185594–43185579 |
| 2PCR | Biot-GTATATTTYGGGTTGGGGGAGG | 55.8 | NT_34772.6 | 5 | 43185718–43185697 | AATAAATAAAACRAAACRACAAATCATCCCC | 53.6 | NT_34772.6 | 5 | 43185643–43185613 |
| Seq primer | ACAACRCCCRAATATTTACATAATT | 45.2 | NT_34772.6 | 5 | 43185670–43185645 | |||||
| CDKN2A | Forward seq | Tm | Reverse seq | Tm | ||||||
| 1PCR | ATGGAGTTTTYGGTTGATTGGT | 56.5 | NT_008413.18 | 9 | 21964802–21964781 | CCCRCCATCCCCTACTCC | 55.2 | NT_008413.18 | 9 | 21964655–21964638 |
| 2PCR | ATGGAGTTTTYGGTTGATTGGT | 56.5 | NT_008413.18 | 9 | 21964802–21964781 | Biot-CCCTCTACCCACCTAAAT | 56.3 | NT_008413.18 | 9 | 21964682–21964665 |
| Seq primer | GGAGTTTTAGGTTGATTGGTT | 38.1 | NT_008413.18 | 9 | 21964800–21964780 | |||||