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. 2011 Dec 2;2:83. doi: 10.3389/fendo.2011.00083

Figure 3.

Figure 3

Screening transcriptional activities of estradiol (E2), estrone (E1) and lumiestrone (LE) using estrogen receptor-dependent reporter gene assays in vitro. ER-negative U251-MG glial cells were transfected with zebrafish ERα ((A)), ERβ1 (B), and ERβ2 (C) and exposed to estrogens or the dimethyl sulfoxide (DMSO) control. In (D) ERα and ERβ-positive human breast cancer MCF-7 cells were used. Data are presented as mean + SEM of luciferase activity relative to β-gal (n = 3). Data were tested for normality and homoscedasticity, and were transformed when appropriate prior to analysis by one-way ANOVA (treatment; p < 0.001) followed by Tukey’s post hoc test (S-Plus, v8.0). a,b,c,dMeans labeled with different superscripts are significantly different (p < 0.05).