Figure A3.
High-pressure liquid chromatography chromatograms of estrone solutions (10 μM) irradiated by sunlight at different times. (A) t = 0, (B) t = 15, (C) t = 60, (D) t = 120, and (E) t = 180 min. In order to simulate environmental conditions estrone was dissolved in untreated sewage water and irradiated by sunlight in Napoli (Italy) in July 2007. HPLC analysis was performed under isocratic conditions (H2O/CH3CN 1:1) on an Agilent 1100 Binary HPLC pumps system equipped with a UV-detector. The column used was a reverse-phase Gemini 5 μ C-18 (250 × 4.60 mm). The detector was set at 220 nm.