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RT-PCR analysis of PG-derived PBANR variants. RT-PCR was performed using B. mori, P. separata, H. armigera, and H. zea PG cDNAs with either (A) a conventional DNA polymerase (Ex Taq) or (B) a GC-optimized DNA polymerase (KOD-FX). Amplifications were performed using 26–32 PCR cycles as indicated.
