Figure 1.

Hs578T cells were seeded in six-well plates at 0.1 × 10⁶ cells in GM followed by 24 h in SFM prior to pre-dosing (A) with SPD (10 ng/ml) or (B) IGFBP-5 ± KT5720 (1 μM). The cells were then re-dosed with IGFBP-3 or IGFBP-5 (each at 100 ng/ml) for a further 24 h in the presence or absence of an apoptotic dose of C2-ceramide. The graphs represent changes in cell death and show the mean ± SEM of three experiments each repeated in triplicate.