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. 2011 Oct 10;2:43. doi: 10.3389/fendo.2011.00043

Figure 4.

Figure 4

Mass-spectrometric determination of sex steroids (A,B) and corticosteroids (C,D) in the male hippocampus. LC-MS/MS chromatograms and steroid derivatives of (A) E2 and (C) CORT. CORT was extracted from the hippocampus of ADX rats. Shaded portions indicate the intensity of the fragmented ions of 17β-E2-pentafluorobenzoxy-picolinoyl [m/z = 339, (A)] and CORT [m/z = 121, (C)], respectively. The vertical axis indicates the intensity of the fragmented ions. The horizontal axis indicates the retention time of the fragmented ions. (B) No effect of castration on hippocampal E2 level as well as no correlation between plasma T and hippocampal E2. These data suggest that hippocampal E2 is mainly produced from hippocampus-synthesized T. (D) Diurnal change of the concentration of CORT in the cerebrospinal fluid (CSF) from the cisterna magna in freely moving rats. The black bar indicates a dark period during which the activity of rats is high. During a dark period, CORT concentration elevates to three- to five-fold of that during a light period. Modified from Hojo et al. (2009), Higo et al. (2011).