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Tracing of dendrites and detection of spines by Spiso-3D of confocal images. Spiso-3D detects spines by geometric calculation. (A) Original image of dendrite. (B) Traced dendrite (red circles) and spines (yellow circles) superimposed on the original image. (C) Calculated diameters of spines are superimposed on the original spine images. Modified from Mukai et al. (2011).
