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. 2011 Oct 10;2:43. doi: 10.3389/fendo.2011.00043

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Copyright © 2011 Hojo, Higo, Kawato, Hatanaka, Ooishi, Murakami, Ishii, Komatsuzaki, Ogiue-Ikeda, Mukai and Kimoto.

This is an open-access article subject to a non-exclusive license between the authors and Frontiers Media SA, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and other Frontiers conditions are complied with.

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Effects of androgens and estrogens on changes in the density and morphology of spines. Spines were analyzed by Spiso-3D along the secondary dendrites in the stratum radiatum of CA1 pyramidal neurons. A 2-h treatment in ACSF without hormone (Control), with 10 nM DHT, with 10 nM T, with 1 nM E2. (A) Density of three subtypes of spines treated with DHT, T, and E2. From left to right, ACSF without hormones (black), 10 nM DHT (blue), 10 nM T (green), and 1 nM E2 (pink). (B) Histogram of spine head diameters. After a 2-h treatment in ACSF without steroids (Control, black), E2 (pink), T (green), and DHT (blue). (C) Total spine density. Vertical axis is the average number of spines per 1 μm of dendrite. Modified from Mukai et al. (2011).