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. 2011 Oct 10;2:43. doi: 10.3389/fendo.2011.00043

Figure 9.

Figure 9

Rapid modulation of LTD by 17β-E2 in hippocampal slices from adult male rats. (A) Time-dependence of maximal EPSP amplitude in CA1. E2 concentration was 0 nM (open circle), 10 nM (red closed diamond), 100 nM PPT (yellow closed triangle), and 100 nM DPN (blue closed square), respectively. Here, 100% EPSP amplitude refers to the EPSP value at t = −40 min prior to NMDA stimulation, irrespective of the test condition. LTD was induced by 30 μM NMDA perfusion at time t = 0–3 min (closed green bar above the graph). Hatched bar above the graph indicates period of time during which E2 was administered. (B) Custom-made 64 multielectrode probe (MED64, Panasonic, Japan) with the hippocampal slice. Stimulation (red circle) and recording (blue circle) electrodes are indicated. (C) Comparison of modulation effect of 17β-E2 and agonists on LTD in CA1. Vertical axis is relative EPSP amplitude at t = 60 min, where EPSP amplitude of the slice without drug application (control) is normalized as 100%. From left to right, the group applied 17β-E2, PPT (ERα agonist) and DPN (ERβ agonist) at indicated concentration. Statistical significance was calculated at 60 min by ANOVAs (*p < 0.05; **p < 0.01). Modified from Mukai et al. (2007), Hojo et al. (2008).