Table 6.
Effect of Gelatin Addition, With and Without methionine, DMSO, and Pluronic F68, on the Process-Associated Loss, Residual Water Content, and Glass Transition Temperature (Tg) of F. tularensis Following Foam Drying (n = 2 Replicates per Data Point or # of Experiment, Except for Residual Water Content and Tg Analysis, for Which n = 3)
| Excipients | Fr 3 | Fr 4 | Fr 5 | Fr 6 | Fr 7 |
|---|---|---|---|---|---|
| Trehalose ( %,w/v) | 30 | 30 | 30 | 30 | 30 |
| Gelatin (%,w/v) | 5 | 5 | 5 | 5 | |
| Methionine (%,w/v) | 2 | ||||
| DMSO (wt %) | 0.5 | ||||
| Pluronic F68 (wt %) | 0.02 | ||||
| Potassium phosphate (mM) | 25 | 25 | 25 | 25 | 25 |
| pH | 8 | 8 | 8 | 8 | 8 |
| Process loss (log10 CFU) | 1.0 ± 0.2 | 1.0 ± 0.7 | 0.7 ± 0.5 | 0.7 ± 0.3 | 1.0 ± 0.2 |
| Residual Water (%) | 5.2 ± 0.1 | 2.5 ± 0.1 | 1.2 ± 0.1 | 1.2 ± 0.3 | 2.6 ± 0.3 |
| Tg (°C) | 57.6 ± 1.3 | 99.4 ± 2.2 | 103.5 ± 1.6 | 103.3 ± 2.8 | 90.9 ± 1.4 |