. Author manuscript; available in PMC: 2012 Aug 1.

Published in final edited form as: J Pharm Sci. 2011 Apr 13;100(8):3076–3087. doi: 10.1002/jps.22563

Table 8.

Virulence of Live Vaccine Strain LVS Samples Administered Intraperitoneally in Mice. Samples included freshly prepared LVS, foam-dried LVS (Fr6 and Fr7), and control (1% gelatin in PBS), administered at several dose levels. Mice were monitored daily for signs of disease for 28 days.

Strain	Dose (CFU)	# Moribund^a/Total	Time to Moribundity (days)
LVS	95	4/4	4, 4, 5, 5
	950	4/4	4, 4, 4, 4
	9500	4/4	4, 4, 4, 4
Fr 6	90	3/4	4, 4, 5
	900	4/4	4, 4, 4, 4
	9000	4/4	4, 4, 4, 4
Fr 7	80	2/4	4, 4
	800	4/4	4, 4, 4, 4
	8000	4/4	4, 4, 4, 4
PBS	–	0/3	–

Moribundity was determined by the exhibition of any three of the following clinical signs: lack of grooming (ruffled fur), hunched gait, lethargy, and closed eyes.