Figure 7.

The gene delivery capability of the triblock copolymer were investigated in both B16-F10 and 293T cell lines. The copolymer used was mPEG–PCL-g–PEI with the highest transfection efficiency at a composition of 5000-2000-2000 (A–D and F). (A) Fluorescence microscopy image of 293T cells; (B) white light image in 293T; (C) fluorescence microscopy image in B16-F10 cells; (D) white light image in B16-F10 cells; (E) agarose gel electrophoresis of mPEG–PCL-g–PEI/DNA complexes using three types of composition at various M/M ratios (A is the triblock copolymer with a composition of 2000-2000-2000, while B is 2000-6000-2000, and C is 5000-2000-2000); and (F) percentage of green fluorescence protein expression in B16-F10 cells.

Abbreviations: PEG, polyethylene glycol; PCL, poly ɛ-caprolactone; PEI, polyethylenimine.