Figure 4.

In vivo circulation time of HFt-based nanoparticles. HFt nanoparticles have been quantified by Western blot using an anti-MSH (bottom) and anti-HFt (top) antibody (see Materials and methods section). Lane 1, reference proteins HFt-MSH-PEG (bottom) and HFt (top), 1 mg/mL; lane 2, empty; lane 3, plasma before HFt-nanoparticle injection; lanes 4–8, plasma at 3 minutes and 1, 3, 6, and 24 hours following intracardiac injection.

Abbreviations: HFt, human protein ferritin; PEG, polyethylene glycol; MSH, melanocyte-stimulating hormone peptide.