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. 2012 Mar 26;7:1613–1621. doi: 10.2147/IJN.S30104

Figure 3.

Figure 3

(A) The uptake intensity of MCF-7 cells after treatment with ACPP-DOX conjugate in the absence (−) or presence of extra collagenase IV at concentrations of 20 μg/mL and 100 μg/mL. (B) The uptake intensity of MCF-7 cells after treatment with ACPP-DOX conjugate in the absence (−) or presence of extra MMP-2 at concentrations of 20 ng/mL and 200 ng/mL. (C) Cellular uptake intensity–time curve of DOX and ACPP-DOX in MCF-7 and HT-1080 cells at different incubation times. (D) Confocal microscopy images of ACPP-DOX or DOX incubated with HT-1080 and MCF-7 cells at 37°C for 0 hour–8 hours.

Notes: Red represents fluorescence of DOX. Blue represents fluorescence of Hoechst 33258 for nuclei staining. Scale bars: 100 μm. *P < 0.001; #P < 0.05; ns = no significance.

Abbreviations: DOX, doxorubicin; ACPP-DOX, activable cell-penetrating peptide–doxorubicin; MMP-2, matrix metalloproteinase-2.