Figure A3.
Curcumin-loaded magnetic nanoparticle formulation increases MDA-MB-231 cell death. Cells (2 × 105) were seeded in six-well plates and treated for 24 hours with 2.5, 5, and 7.5 μM of curcumin or curcumin-loaded magnetic nanoparticles. The cells were collected both live and dead (floating) and incubated at room temperature in the dark with 5 μL propidium iodide for 20 minutes in 2 mL medium containing hydroxyethyl piperazineethanesulfonic acid without phenol red. After completion of the incubation period, propidium iodide stain was quantified for 5000 cells using an Accuri® C6 flow cytometer (BD Accuri Cytometers, Ann Arbor, MI) in the FL2 channel. The experiment was conducted in triplicate.
Abbreviations: CUR, curcumin; MNP-CUR, curcumin-loaded nanoparticles; PI, propidium iodide.