Figure 1.

Fabrication and characterization of formyl peptide receptor-targeting nanoprobes and in vitro study to assess the specificity of formyl peptide receptor-targeting nanoprobes to activated neutrophils. (A) Schematic illustration of formyl peptide receptor-targeting nanoprobes. (B) Absorbance measurements of peptides, formyl peptide receptor-targeting nanoprobes, and control nanoprobes. (C) Excitation and emission spectra of formyl peptide receptor-targeting nanoprobe. (D) Fluorescence microscopy images of activated neutrophils incubated with formyl peptide receptor-targeting nanoprobe (red color) and neutrophil-specific antibody (green color) and their superimposed image. (E) Correlation between neutrophil numbers and neutrophil-associated fluorescence intensities following incubation with either formyl peptide receptor-targeting nanoprobe or control probe.

Abbreviations: cFLFLF, cinnamoyl-Phe-(D)Leu-Phe-(D)Leu-Phe; EDC, 1-Ethyl-3-(3-(dimethylaminopropyl)carbodiimide; FPR, formyl peptide receptor; NH₂, amine; PEG, polyethylene glycol; R², correlation coefficient.