Table 1.

Target cDNA primer sequences used in quantitative polymerase chain reaction

VEGFA

Forward: 5′-GAGCCTTGCCTTGCTGCTCTAC-3′

Reverse: 5′-CACCAGGTCTCGATTGGATG-3′

PCR product size: 148 bp

ITGB

Forward: 5′-TGTGTCAGACCTGCCTTGGTG-3′

Reverse: 5′-AGGAACATTCCTGTGTGCATGTG-3′

PCR product size: 105 bp

ICAM1

Forward: TGAGCAATGTGCAAGAAGATAGC

Reverse: CCCGTTCTGGAGTCCAGTACA

PCR product size: 105 bp

LAMA1

Forward: TGGTAACATTTGCCACCACGA

Reverse: TTGCCTCCGATCAGCATGAC

PCR product size: 127 bp

GAPDH (housekeeping gene)

Forward: 5′-GCACCGTCAAGGCTGAGAAC-3′

Reverse: 5′-TGGTGAAGACGCCAGTGGA-3′

PCR product size: 138 bp

Abbreviations: GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ICAM1, intercellular adhesion molecule 1; ITGB, β-integrin; LAMA1, laminin-1; VEGFA, vascular endothelial growth factor A.